Want to know if you’re seeing something new? Open the finished assembly in SeqMan Ultra to view known and novel transcripts separately in two highly customizable and sortable reports.Īccording to the study authors, SeqMan NGen “produced both annotated and novel transcripts lists.
Lasergene Genomics supports many options for downstream analysisĪfter de novo transcriptome assembly, other applications in the Lasergene Genomics package allow different types of downstream analysis. The comparison study found that SeqMan NGen “…clearly defines excluded reads in its project report…” By contrast, SeqMan NGen reports which reads were excluded.
Clc genomics workbench free trial software#
Software that lacks the ability to report excluded reads may be oversampling the reads, reducing the precision of the transcriptome assembly. SeqMan NGen reports whether contaminant sequences were present The total count of transcript fragments that aligned and matched RefSeq sequences provides the sequencing coverage. Many data sets assembled with SeqMan NGen produce a large number of long transcripts that are likely full-length transcripts. How does SeqMan NGen do it? SeqMan NGen automatically attempts to group contigs from the same gene, and then name and annotate them based on the best match to a collection of annotated reference sequences (the “Transcript Annotation Database”) extracted from data on NCBI’s RefSeq website. The study authors noted that “ … the Lasergene SMN Trace Evidence consensus-calling algorithm generated longer contigs on average…Meanwhile, CLC GW had assembled over nine times the amount of contigs…” Using its proprietary assembly algorithm, however, SeqMan NGen creates fewer and longer contigs than CLC Genomics Workbench.
Performing meaningful downstream analysis on this many unannotated contigs is nearly impossible. With other applications, de novo assembly of RNA-Seq data can potentially result in thousands of unlabeled contigs representing the expressed transcripts. SeqMan NGen assembly output contains fewer and longer contigs
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